Analyzing the weak dimerization of a cellulose binding module by analytical ultracentrifugation

Analytical ultracentrifugation of colloids.

In this contribution the use of Analytical Ultracentrifugation (AUC) for the modern analysis of colloids is reviewed. Since AUC is a fractionation technique, distributions of the sedimentation coefficient, particle size and shape, molar mass and density can be obtained for particle sizes spanning the entire colloidal range. The Ångström resolution and the reliable statistics with which particle...

Determination of Spectrophotometric Absorptivity by Analytical Ultracentrifugation

Rapid determination of the absorptivity for a recombinant IgG monoclonal antibody using the Beckman equipped with both Raleigh interference and UV absorbance optical systems. The analytical ultracentrifuge data for determining spectrophotometric absorptivities is compared to experimental data from quantitative amino acid analysis and an enzymatic digestion method.

Analytical ultracentrifugation of latexes

Analytical Ultracentrifugation (AUC) is a fractionating technique based on the synchronized optical detection of the concentration profiles of the sample under a centrifugal acceleration of up to 300,000 g. The skilled choice of solvent, rotational speed and detection optics enables a variety of complementary AUC experiments due to the controlled interplay of centrifugal force, hydrodynamic fri...

Characterizing RNA Nanoparticles by Analytical Ultracentrifugation

Chad T. Schwartz, Daniel Jasinski, and Peixuan Guo* Beckman Coulter, Inc., Indianapolis, IN 46268 University of Kentucky, Department of Pharmaceutical Sciences,

What is Analytical Ultracentrifugation?